How to do the effect evaluation test of air disinfectant in 20m bacterial aerosol chamber?

The new crown epidemic is not over yet, our living environment needs to be disinfected frequently, and whether the performance of air disinfectant meets the requirements needs to be tested by neutralization identification test. The following air disinfectant neutralization effect evaluation test is suitable for the test of 20m~30m bacterial aerosol chamber. Let's find out together.

A. Preparation of tools

Test Product: Air Sanitizer

Test equipment: 20~30m³ bacterial aerosol chamber/20m~30m bacterial aerosol chamber

Equipment model: HYKJ-20/HYKJ-30

Manufacturer: Huanyi Instrument

B. The steps of the air disinfectant neutralization identification test:

This method is suitable for the disinfection effect evaluation test of 20 m~30 m aerosol chamber, and the sampler is a six-stage sieve air impact sampler.

1. Preparation of bacterial suspension

Diluted with nutrient broth to obtain a bacterial suspension for the test with a concentration of 5×10CFU/mL~3×10^4CFU/mL

2. The neutralizer identification test is divided into three groups

Group 1: Draw 0.1 mL of the bacterial suspension for the test respectively, spread it evenly on two nutrient agar plates containing neutralizer, and count the viable bacteria culture. Observe whether the neutralizer has an inhibitory effect on the growth of the test bacteria.

Group 2: In accordance with the dosage of disinfectant required by the instructions, spray in a 20 m ~ 30 m bacterial aerosol chamber, immediately sample with a six-stage air sieve impact sampler containing a neutralizer nutrient agar plate (the air sampling volume is the same as the pre-sterilized sampling volume in the air disinfection identification test) for 10 minutes.

Draw 0.1 mL of the bacterial suspension for the test and apply it to six plates with disinfectant to count the viable bacteria (the average of the colony counts of the six plates is taken for the calculation of the results).

Group 3: Draw 0.1 mL of the bacterial suspension for the test respectively, spread it evenly on the surface of two ordinary nutrient agar plates, and count the viable bacteria.

The above is the steps for the neutralization and identification test of air disinfectant in the bacterial aerosol chamber sorted out by the instrument team of Huanyi Instrument. If you have any questions, you can send a private letter to the editor, or visit the "Huanyi Instrument" official website for relevant information.